Maekoroskoupo ea fluorescence ke mofuta oa microscope ea optical e sebelisang mohloli oa khanya e matla haholo ho bonesa mohlala le ho hlasimolla li-fluorochromes tsa sampole. Hangata khanya ea mohlala e etsoa ka mohloli o khanyang o hlahisang leseli la ultraviolet. Li sebelisoa haholo libakeng tsa baeloji, tsa bongaka le tsa indasteri.
microscope ea fluorescence e sebetsa joang?
Sebonela-hōle sa fluorescence se sebelisa lebone la mercury kapa xenon ho hlahisa leseli la ultraviolet. Leseli le tla ka har'a microscope 'me le otla seipone sa dichroic - seipone se bonahatsang mefuta e mengata ea maqhubu 'me se lumella mofuta o mong ho feta. Seipone sa dichroic se bonahatsa leseli la ultraviolet ho ea ho mohlala. Mehlala e meng ea fluoresce ka tlhaho tlas'a leseli la ultraviolet hobane e na le lintho tsa fluorescent tse kang chlorophyll. Haeba mohlala o lokelang ho shejoa ha o na fluoresce ka tlhaho, o ka silafatsoa ka lidae tsa fluorescent tse bitsoang fluorochromes.
microscope ea fluorescence e kenyelletsa eng?
1. Mohloli oa Leseli
Mehloli e atileng haholo ea leseli ke mercury, xenon, le li-LED. Mercury e fana ka boleng bo botle ba khanya bakeng sa microscope ea fluorescence. Li-LED li ntse li tsebahala haholo hobane li theko e tlase ho feta mehloli e meng 'me li sebelisa matla a fokolang.
| Lebone la Mercury | Lebone la Xenon | LED | |
| Wavelength of Emitted Light | 350-370 nm | 400nm ~ 450nm | 400-700 nm |
| Bophelong bohle(hora) | 200-300 | 400-600 | 10000 |
| Molemo | 1. Leseli le Phahameng ka ho Fetisisa, Mehloli e Tloaelehileng ea Leseli. 2. E hlahisa ultraviolet e matla le blue-violet ho thabisa mefuta eohle ea fluorophores. 3. Litšoantšo tse khanyang le tse mebala-bala. | 1. Matla a spectral a tsitsitse. 2. Matla a matla a spectral ho infrared le mid-infrared. | 1. Ho bonolo ho nkela sebaka. 2. Ha ho hlokahale ho futhumatsa. 3. E ka bulela le ho tima hang-hang. 4. Laola matla a mohloli oa leseli. |
| Bothata | 1. Nako e Khutšoane ea Bophelo. 2. Nako e telele ea ho futhumatsa. | 1. Lebokose le khethehileng la motlakase oa DC le hlokahalang bakeng sa mocheso o feteletseng. 2. E theko e boima ho feta Lebone la Mercury | 1. Leseli la UV le fokola ho feta lebone la mercury. 2.Bophahamo bo bong le bo bong ba khanya bo hloka LED e arohaneng ka lebaka la li-bandwidth tse patisaneng tseo li li ntšang. |
Mercury Fluorescent Attachment
Sehokelo sa Fluorescent ea LED
2. Sefe ea Phallo
Sesefa se hlasimollang se bohlokoa bakeng sa ts'ebetso ea microscope ea fluorescence. E fetisa khanya ea bolelele bo bokhutšoane ba maqhubu, boo dae ea fluorescent e ka monyang. Hape, e thibela mehloli e meng ea leseli le thabisang.
Setlhopha sa Tlhaselo(B,G,U,V)
3. Seipone sa Dichroic
Seipone sa dichroic ke mofuta oa filthara ea optical e bonts'ang leseli ka maqhubu a itseng ha e ntse e fetisetsa tse ling. E sebelisoa ka maekerosekoupo ea fluorescence ho arola maqhubu a maqhubu a matla le a ho tsoa.
4. Sefa sa Emission
Emission filter e fetisa feela maqhubu a hlahisoang ke fluorophore 'me e thibela khanya eohle e sa batleheng ka ntle ho sehlopha sena - haholo-holo maqhubu a excitation.
5. Lidae tsa fluorescent
Lidae tsa fluorescent ke metsoako ea lintho tse phelang tse nang le thepa ea fluorescence, eo ka eona li ka etsang setšoantšo sa fluorescent ka ho ntša khanya e tala e bonahalang e fapaneng haholo ka mor'a ho hlasimoloha ke khanya e khanyang ea ultraviolet. Lidae tsa fluorescent tse sebelisoang ka tloaelo ke; DAPI (49,6-diamidino-2-phenylindole), acridine orange, auramine-rhodamine, Alexa Fluors, kapa DyLight 488.
Ke mefuta e mekae ea microscopes ea fluorescence?
1. Maekerosekoupo e otlolohileng ea Epifluorescence:
Ke mofuta o atileng haholo oa microscope ea fluorescence. Ho tsosoa ha fluorophore le ho lemoha ha fluorescence ho etsoa ka tsela e tšoanang ea leseli (ke hore ka sepheo). Boholo ba li-microscopes tsa fluorescence, haholo-holo tse sebelisoang ho saense ea bophelo, ke tsa moralo oa epifluorescence.
BS-2081F Research Fluorescent Biological Microroscope
2. Maekerosekopo ea Confocal Fluorescence:
Confocal Fluorescence Microscope: Mofuta ona oa microscope ea fluorescence o kopanya laser scanning le khanya ea fluorescent ho hlahisa setšoantšo. E ka sebelisoa lits'ebetsong tse fapaneng, joalo ka ho ithuta lisele le lisele, ho bona liprotheine le lintho tse ling ka har'a lisele, le ho metha botenya ba lisebelisoa.
BS-2081F Research Fluorescent Biological Microroscope
3. Maekerosekopo e Inverted Fluorescence
Mofuta ona oa mohloli o khanyang oa microscope le condenser li ka holimo, li shebile tlase. Lehlakore la khanya le tlameha ho ba likhato tse 90 mabapi le bokaholimo ba mohlala o hlahlojoang.
BS-2095F Maekerosekoupo e Inverted Fluorescent
Melemo le mefokolo ea microscope ea fluorescence ke efe?
Melemo:
- E nolofalletsa ho shebella lisele ka har'a lintho tse phelang ntle le ho li senya
- E fana ka litšoantšo tse phahameng tse nang le mebala e nepahetseng
- E lumella ho ithuta ka lits'ebetso tse phelang liseleng
- Sebelisa ho khetholla mefuta e fapaneng ea limolek'hule ka har'a lisele, joalo ka liprotheine kapa nucleic acid.
Mefokolo:
-E lumella feela ho shebella meaho e itseng ka har'a sele e tšoailoeng ka dae ea fluorescent.
-The photobleaching ka lebaka la ho tsosoa ha elektronike nakong ea ts'ebetso ea fluorescence e ka ama limolek'hule tse sebetsang tsa lidae tsa fluorescent. Ka lebaka leo, lidae tse sebetsang li ka 'na tsa lahleheloa ke lik'hemik'hale tsa tsona tse hlahisang matla a tlhahiso ea fluorescence.
-Lisele li ka hlaseloa habonolo ke phello ea phototoxic ka mor'a ho silafatsoa ka dae ea fluorescent, kaha limolek'hule tsa fluorophore li monya li-photon tse matla tse tsoang khanyeng e khutšoanyane ea maqhubu.
Lisebelisoa tsa microscope ea fluorescence ke life?
Li-microscopes tsa Fluorescence li sebelisoa haholo mafapheng a fapaneng a lipatlisiso le ts'ebeliso ho kenyelletsa biochemistry, cell biology, microbiology, immunology le bongaka.
1. Lefapheng la baeloji, microscope ea fluorescence e etsa hore ho be le boitsebiso bo nepahetseng le bo qaqileng ba likarolo le mesebetsi ea lisele tsa cellular le submicroscopic ka thuso ea ho ngola dae ea fluorescent.
2. Lefapheng la bongaka, microscope ea fluorescence e ka sebelisa li-reagents tsa fluorescent ho bona boteng le ho ajoa ha libaktheria le likokoana-hloko, kapa ho thusa ho ngola lipakane tsa ho buoa ho nolofalletsa ho buuoa.
3. Lefapheng la mineralogy, microscope ea fluorescence e atisa ho sebelisoa ho ithuta lintho tse nang le thepa ea fluorescence e ikemetseng, e kang asphalt, petroleum, mashala, graphene oxide le liminerale tse ling.
4. Ho saense ea thepa, microscope ea fluorescence e ka sebelisoa indastering ea masela kapa indasteri ea pampiri ho hlahloba lisebelisoa tse thehiloeng ho fiber.
Nako ea poso: Mar-14-2023