Fluorescence microscopy e fetotse bokhoni ba rona ba ho bona ka mahlo a kelello le ho ithuta mehlala ea baeloji, e re lumellang hore re hlahlobe lefatše le rarahaneng la lisele le limolek'hule. Karolo ea bohlokoa ea microscopy ea fluorescence ke mohloli o khanyang o sebelisetsoang ho thabisa limolek'hule tsa fluorescent ka har'a sampuli. Ho theosa le lilemo, ho ’nile ha sebelisoa mehloli e sa tšoaneng ea khanya, e ’ngoe le e ’ngoe e e-na le litšobotsi le melemo ea eona e ikhethang.
1. Lebone la Mercury
Lebone la mercury le nang le khatello e phahameng, ho tloha ho li-watts tse 50 ho isa ho tse 200, le hahiloe ka khalase ea quartz 'me le na le sebōpeho sa spherical. E na le tekanyo e itseng ea mercury ka hare. Ha e sebetsa, ho tsoa ho hlaha pakeng tsa li-electrode tse peli, ho etsa hore mercury e fetohe mouoane, 'me khatello ea ka hare sebakeng e eketseha ka potlako. Ts'ebetso ena hangata e nka metsotso e 5 ho isa ho e 15.
Ho ntšoa ha lebone le phahameng la khatello ea mercury ho bakoa ke ho arohana le ho fokotseha ha limolek'hule tsa mercury nakong ea ho tsoa ha elektrode, e leng se lebisang ho nts'etsopele ea li-photon tse khanyang.
E hlahisa khanya e matla ea ultraviolet le blue-violet, e etsa hore e tšoanelehe bakeng sa lisebelisoa tse fapaneng tsa fluorescent, ke ka lebaka leo e sebelisoang haholo ho microscopy ea fluorescence.
2. Mabone a Xenon
Mohloli o mong o sebelisoang hangata ho khanya e tšoeu ho microscopy ea fluorescence ke lebone la xenon. Mabone a Xenon, joalo ka mabone a mercury, a fana ka mefuta e mengata ea maqhubu ho tloha ho ultraviolet ho isa haufi le infrared. Leha ho le joalo, li fapane ka ponahalo ea tsona ea thabo.
Mabone a Mercury a tsepamisa mohopolo oa 'ona libakeng tse haufi le mahlaseli a kotsi, a maputsoa le a matala, e leng se netefatsang tlhahiso ea mats'oao a khanyang a fluorescent empa a tla le phototoxicity e matla. Ka lebaka leo, mabone a HBO hangata a boloketsoe lisampole tse tsitsitseng kapa litšoantšo tse fokolang tsa fluorescence. Ka lehlakoreng le leng, mehloli ea lebone ea xenon e na le profil ea ho hlasimolla e bonolo, e lumellang papiso ea matla ho li-wavelengths tse fapaneng. Tšobotsi ena e molemo bakeng sa lits'ebetso tse kang litekanyo tsa mahloriso a calcium ion. Mabone a Xenon a boetse a bonts'a thabo e matla sebakeng se haufi le infrared, haholo-holo ho pota 800-1000 nm.
Mabone a XBO a na le melemo e latelang ho feta mabone a HBO:
① Matla a tšoanang a pono
② Matla a matla a pono sebakeng sa infrared le bohareng ba infrared
③ Phallo e matla ea matla, e nolofaletsang ho fihla moo sepheo sa sona se kenang teng.
3. Li-LED
Lilemong tsa morao tjena, ho hlahile mohanyetsi e mocha sebakeng sa mehloli ea leseli la fluorescence microscopy: LEDs. Li-LED li fana ka monyetla oa ho tima ka potlako ho li-milliseconds, ho fokotsa nako ea ho pepeseha ha sampole le ho lelefatsa nako ea bophelo ea lisampole tse bobebe. Ho feta moo, leseli la LED le bonts'a ho bola kapele le ka nepo, ho fokotsa haholo phototoxicity nakong ea liteko tsa nako e telele tsa lisele tse phelang.
Ha ho bapisoa le mehloli ea mabone a masoeu, li-LED hangata li tsoa ka har'a khanya e fokolang. Leha ho le joalo, ho na le lihlopha tse ngata tsa LED tse fumanehang, tse lumellang lits'ebetso tse fapaneng tsa mebala e mengata ea fluorescence, e leng se etsang hore li-LED e be khetho e tsebahalang haholo ho li-setups tsa sejoale-joale tsa fluorescence microscopy.
4. Mohloli oa Leseli la Lasers
Mehloli ea leseli la laser e na le monochromatic haholo ebile e tataisoa, e etsa hore e be e loketseng bakeng sa microscope ea qeto e phahameng, ho kenyeletsoa le mekhoa ea tharollo e phahameng joalo ka STED (Stimulated Emission Depletion) le PALM (Photoactivated Localization Microscopy). Leseli la laser hangata le khethoa ho ts'oana le bolelele bo ikhethileng ba maqhubu bo hlokehang bakeng sa sepheo sa fluorophore, ho fana ka khetho e phahameng le ho nepahala ho hlasimollang ha fluorescence.
Khetho ea mohloli oa leseli oa microscope ea fluorescence e itšetlehile ka litlhoko tse khethehileng tsa liteko le litšobotsi tsa mohlala. Ka kopo ikutloe u lokolohile ho ikopanya le rona haeba u hloka thuso leha e le efe
Nako ea poso: Sep-13-2023